제공하는 Vector(벡터) 종류

pUC57	pBR322	pBluescript II KS+
pUC57-Kan	pUCm-T	pBluescript II SK+
pUC57m (No Ndel)	pUC19	pBluescript II SK-

그 외 Vector는 추가비용이 발생할 수 있습니다

문의

Gene Synthesis Process

1. Oligonucleotide synthesis

Multiple, long primers (~50-60 bases in length) with similar melting temperature (Tm) are chemically synthesized as fragments based on the desired final gene sequence. Primers are designed to assemble with each other through overlapping sequences.

2. Amplification: PCR

Using PCR, the primer fragments are assembled into blocks of up to 1kb of double stranded DNA.

3. Fragment Assembly

These blocks are then assembled and amplified once again using PCR to create one large double stranded DNA construct.

4. Fragment Sequencing

All genes are verified by DNA sequencing and only those with the correct sequences are selected. Here, Sanger Sequencing is used due to its long read length short run time.

5. Correcting Errors: Fragments

In the event errors are identified, we perform error correction work to correct mutated bases. This ensures that the gene synthesized conforms to the specifications planned in the beginning of the project.

6. Vector Insertion

The synthesized DNA is inserted into a specific vector. The vector is then isolated and amplified. Unless otherwise specified, the default vector used is the pUC57-Amp vector.

7. Selecting Clones

Colonies are grown and appropriate ones are then selected containing the synthetic gene of interest.

8. Sequencing Gene: First Round

All genes are verified by DNA sequencing and only those with the correct sequences are selected. Here, Sanger Sequencing is used due to its long read length short run time.

9. Correcting Errors: Gene

10. Sequencing Gene: Final Round

All genes are verified by DNA sequencing and only those with the correct sequences are selected. Here, Sanger Sequencing is used due to its long read length short run time.